The pUC18 plasmid and pUC19 plasmid enable successful cloning of larger DNA fragments than the M13 mp18 RF Phage Vector. Because these cloning vectors contain a multiple cloning site at the lacZ' region, recombinant plamids can be verified via blue/white colony screening using agar plates containing IPTG and X-Gal. Expression of target DNA is enabled by the presence of a lac promoter in the cloning vectors.
The pUC18 plasmid and pUC19 plasmid are suitable for dideoxy DNA sequencing using M13 primers. The Deletion Kit for Kilo-Sequencing may be used to easily sequence pUC18 Plasmid and pUC19 Plasmid containing large DNA insertions.