PrimeSTAR HS DNA Polymerase, the original enzyme in the PrimeSTAR series, is a novel PCR enzyme that provides high fidelity and accuracy during amplification of large products (8.5 kb for human genomic DNA; 22 kb for lambda DNA). This hot start PCR enzyme can be useful during highly demanding cloning (e.g., amplification of cDNA libraries) and sequencing applications. The fidelity of PrimeSTAR HS DNA Polymerase has been calculated by direct sequencing analysis. Such analysis provides a more realistic estimate of the actual frequency of incorporation errors compared with other commonly-used indirect phenotypic analyses such as lacl. Calculation methods such as lac1, which are often used by other enzyme suppliers, are prone to error due to inherent lethal and silent mutations.
PrimeSTAR HS DNA Polymerase also offers excellent amplification efficiency and a short reaction time. Because PrimeSTAR HS is a hot start PCR enzyme, false initiation events during reaction assembly are minimized, reducing experimental background.
PrimeSTAR HS DNA Polymerase possesses extremely high priming efficiency. Therefore, highly specific amplifications can be achieved using short annealing times of just 5-15 seconds.
PrimeSTAR HS (Premix): The PrimeSTAR HS (Premix) is an optimized mixture composed of 2X PrimeSTAR HS DNA Polymerase, reaction buffer and dNTP mixture. This is useful for high-throughput applications because the 2X premixed reaction format reduces contamination risk.
Note regarding fidelity comparisons: Takara uses sequencing analysis to determine incorporation error rate because most researchers who require high fidelity perform the following reactions: PCR, cloning and sequencing. Determination of error rate by direct sequence analysis, therefore, corresponds closely to the method that most researchers would use during their own applications. Please note that values obtained using different fidelity measurement methods (e.g., direct sequencing, Kunkel method, Cline method, lacI method) do not yield directly comparable data.